| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124656 |
| Chromosome: | chromosome 1 |
| Location: | 2833119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017050 | MCP1 | (1 of 22) IPR018108//IPR023395 - Mitochondrial substrate/solute carrier // Mitochondrial carrier domain; Mitochondrial substrate carrier protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTTTCCTCCGAACACGTTTTACACGCAC |
| Internal bar code: | GTCGGATCCGGTGACGAAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 99.6788 |
| LEAP-Seq n confirming: | 28547 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 139 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAAACTGATGTTCTTGA |
| Suggested primer 2: | TTGCTGTTTTTGTGCTGAGG |