| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124669 |
| Chromosome: | chromosome 7 |
| Location: | 958681 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319300 | GWD1 | Alpha-glucan water dikinase 1; (1 of 1) K08244 - alpha-glucan, water dikinase (E2.7.9.4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTAAGGCGCAGCACGTGTTGGGTTCAG |
| Internal bar code: | CCTTTAGAGTTATTGTAACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 98.4615 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTGCTGAAGGAGAGAC |
| Suggested primer 2: | TGCCGACGAGTCTAGAAGGT |