| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124685 |
| Chromosome: | chromosome 3 |
| Location: | 6196257 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAGCGGCTCCACAGCCTACACGCCCGG |
| Internal bar code: | ATACATCGGCAGGTCGTCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 99.7768 |
| LEAP-Seq n confirming: | 447 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCTTGTACTGCCCTGCT |
| Suggested primer 2: | ACACACACCTGCTTCTGTGC |