Insertion junction: LMJ.RY0402.124690_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.124690
Chromosome:chromosome 2
Location:4858510
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g103250 MRP5 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTGTCTCGGTGCCTTCCGGTCCCCACGG
Internal bar code:CCCCATAGAATAGGGTGTGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:139
LEAP-Seq percent confirming:96.3707
LEAP-Seq n confirming:1248
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAATAAGTGGTGCCGTTCCT
Suggested primer 2:CCTGTTCCTCACTGCTCCTC