| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124698 |
| Chromosome: | chromosome 7 |
| Location: | 3644538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAATATACAAGCAGCGCACGCGACTGC |
| Internal bar code: | TTCAATCGCTGGGGTATATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 99.7864 |
| LEAP-Seq n confirming: | 4204 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAGCTTCGCAAAGGAC |
| Suggested primer 2: | GTCCGGTGAGTTCCTGATGT |