| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124700 |
| Chromosome: | chromosome 12 |
| Location: | 7643415 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554700 | SEC22 | Qb-SNARE protein, Sec20-family; (1 of 1) K08517 - vesicle transport protein SEC22 (SEC22) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGTGGCAGGCTGGTGGTGTGGGGCGGT |
| Internal bar code: | CCGCCGATGGTGGGAAACGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 99.8546 |
| LEAP-Seq n confirming: | 2747 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTGGTTTTCACACGAAC |
| Suggested primer 2: | AGTGATGCTTGGAACCCTTG |