| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124707 |
| Chromosome: | chromosome 10 |
| Location: | 6114939 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463700 | (1 of 1) PF09184 - PPP4R2 (PPP4R2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGGCGGCTGGCTTTTCCATGGCTGTCA |
| Internal bar code: | CATGCATGGGGACAACCGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 96.2134 |
| LEAP-Seq n confirming: | 559 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGGTTTATGGAGCCAAAC |
| Suggested primer 2: | GCACACCTTGTTCTCAGCAG |