| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124712 |
| Chromosome: | chromosome 10 |
| Location: | 5284407 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457400 | (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGCTGGTGCAGTGTTGCAGCCCACGCA |
| Internal bar code: | TGACGTGGAGGGTTGAAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 98.2143 |
| LEAP-Seq n confirming: | 1100 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAACCTGGTTTTGCCTGT |
| Suggested primer 2: | CAGATGGTTGCTGAACATGG |