| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124724 |
| Chromosome: | chromosome_6 |
| Location: | 7600726 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g301150 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAATGGATTCGACCGGCACAAACCAAGGT |
| Internal bar code: | AGACTCAGGGGGGAGGTGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 99.7106 |
| LEAP-Seq n confirming: | 1723 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATACAGCCGAGACACCC |
| Suggested primer 2: | GAGGTGTTGTGGGAAGTGGT |