| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124725 |
| Chromosome: | chromosome 12 |
| Location: | 4883537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525300 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCCTCCCCCTGATGAACCGTGTTCCC |
| Internal bar code: | CGCAATGAAGATTGAATACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 99.8083 |
| LEAP-Seq n confirming: | 3644 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTTCAGCAGGTGTGAT |
| Suggested primer 2: | AGTGCTTGAGTGTGACACGG |