Insertion junction: LMJ.RY0402.124726_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.124726
Chromosome:chromosome 17
Location:2098456
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g712250 (1 of 4) 2.7.11.13 - Protein kinase C / PKC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACGTGGAGACGATAGTACATAGCACGC
Internal bar code:TTTTTTGCTGTGTTAGTTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:349
LEAP-Seq percent confirming:99.4505
LEAP-Seq n confirming:5430
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCAGTAGGCAGACGTA
Suggested primer 2:GACAAATTACACGGTCGCCT