| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124745 |
| Chromosome: | chromosome 14 |
| Location: | 1132521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615400 | THB1 | Truncated hemoglobin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTACTAGCAATGCCCGACCCCGCCAGC |
| Internal bar code: | GCGGATTCCGTACCTCGCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.7671 |
| LEAP-Seq n confirming: | 5569 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACACGCCTCCTTACACCC |
| Suggested primer 2: | GGTGGTAGCGAAGCTAGGGT |