| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124753 |
| Chromosome: | chromosome 11 |
| Location: | 3671143 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482483 | (1 of 1) K12599 - antiviral helicase SKI2 (SKI2, SKIV2L) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCCGCAGTTGGGCGTGTGTACAACTTC |
| Internal bar code: | CCTGTAGAAATTCAGCGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 47.563 |
| LEAP-Seq n confirming: | 283 |
| LEAP-Seq n nonconfirming: | 312 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGCAGCTCATGTGGAGG |
| Suggested primer 2: | GTTTGGATAGCTGGGAGGGT |