| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124755 |
| Chromosome: | chromosome 4 |
| Location: | 3101419 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225700 | (1 of 1) IPR023401 - Ornithine cyclodeaminase, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACAGCCATCACCGCGACGCCTCTTCC |
| Internal bar code: | TCATACTGCCGTAGCCGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 55.5673 |
| LEAP-Seq n confirming: | 4751 |
| LEAP-Seq n nonconfirming: | 3799 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCGGTTTTACTGGATGT |
| Suggested primer 2: | ATGAAGGTCGATTTGATCCG |