Insertion junction: LMJ.RY0402.124773_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.124773
Chromosome:chromosome 16
Location:6012207
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g676450 CPLD39 Conserved in the Plant Lineage and Diatoms 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGCAAATATGACCACTGATCGGGTGGGA
Internal bar code:TCAATTCCAAGGCTTCGGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGGACGGAGCTGGACTTG
Suggested primer 2:AATAAGGTGAGGTGGCGTTG