| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124806 |
| Chromosome: | chromosome 10 |
| Location: | 5511232 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459200 | PMA2,ACA4 | (1 of 3) K01535 - H+-transporting ATPase (E3.6.3.6); P-type ATPase/cation transporter, plasma membrane | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGGCTTGACGAGGTGAGCTCAGAGCC |
| Internal bar code: | CTGTCTTCGGCATGGGACCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.4966 |
| LEAP-Seq n confirming: | 593 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTAGCCGAGGAACACC |
| Suggested primer 2: | AATAGTGGAGGTGGGTGCTG |