| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124848 |
| Chromosome: | chromosome_1 |
| Location: | 1723784 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009250 | TOP2 | DNA topoisomerase II | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACGAGGCCTAAGCTGCGCGTCCTATCACT |
| Internal bar code: | CCGCGTGTGGCGACTGGAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGAGAAGAAGCCCAAG |
| Suggested primer 2: | ACCCTACCTACGCTCCCACT |