| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124862 |
| Chromosome: | chromosome 14 |
| Location: | 2547029 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625600 | FHL10 | (1 of 2) PF02010 - REJ domain (REJ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAAGGGGCGGCCCTTGAACCTACCTGCA |
| Internal bar code: | TAGTAAAGCCAGGTTCAATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 99.6958 |
| LEAP-Seq n confirming: | 2950 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATAGTCGTGGGTGCAGT |
| Suggested primer 2: | CACACCACGTGCTGGTTTAC |