| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124897 |
| Chromosome: | chromosome 3 |
| Location: | 6351835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193750 | GCST,GCST1 | Glycine cleavage system, T protein; (1 of 1) K00605 - aminomethyltransferase (gcvT, AMT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTATAATACAAGCCTCCTACTCATCCC |
| Internal bar code: | GCACGTCGGGGTCGCTCAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1055 |
| LEAP-Seq percent confirming: | 99.7119 |
| LEAP-Seq n confirming: | 5538 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTGTGTGTGATGGTGTT |
| Suggested primer 2: | GTGGTGACAAAGATGCCCTT |