| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124925 |
| Chromosome: | chromosome 1 |
| Location: | 3957669 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025767 | (1 of 2) PF08457 - Sfi1 spindle body protein (Sfi1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGCAGCCTTACCGGTACGCACGCCGAC |
| Internal bar code: | GCAATAGCTACCAACAGCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 99.88 |
| LEAP-Seq n confirming: | 1664 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGATGAAGCGGTGGAATC |
| Suggested primer 2: | CTGTCACCGCCTGCAGTAGT |