| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124943 |
| Chromosome: | chromosome_12 |
| Location: | 6135840 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536000 | ALA2 | ATPase, aminophospholipid transporter | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCATGATGTGTTGCCCTTCGCCTTAGCTC |
| Internal bar code: | GTTAGAAGCTTGCGCTGCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 99.1714 |
| LEAP-Seq n confirming: | 9216 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGGTTGTTGATTTCCTT |
| Suggested primer 2: | GGACAAGTTTAGGGGTGCAA |