| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124953 |
| Chromosome: | chromosome 3 |
| Location: | 6639133 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196900 | (1 of 1) PTHR10809:SF6 - AT11025P-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAAATTAAGTTATGGACACGCGTTCCTG |
| Internal bar code: | GGCGGTTTAGTGATATATCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 91.8828 |
| LEAP-Seq n confirming: | 1630 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTTGGCGTTATTTGCAC |
| Suggested primer 2: | GAACCAGCAGCTCAAAGGAC |