| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124961 |
| Chromosome: | chromosome 7 |
| Location: | 1570256 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324550 | LDH1,LDH,HPR4 | (1 of 1) 1.1.1.28 - D-lactate dehydrogenase / D-lactic dehydrogenase; D-lactate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGAGCAGGCAGGGCGCCAGCAGAGCAC |
| Internal bar code: | CCTGCCAAGTGTTGCATTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 719 |
| LEAP-Seq percent confirming: | 99.2616 |
| LEAP-Seq n confirming: | 941 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCAAGGTGTTTGCCTAT |
| Suggested primer 2: | CGATGTTCTGAATGGTGGTG |