| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124965 |
| Chromosome: | chromosome 17 |
| Location: | 3475764 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724950 | (1 of 1) PTHR16161:SF0 - TRANSCRIPTIONAL PROTEIN SWT1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGACCGCCGTGCTATCGTGCTGCCAGAG |
| Internal bar code: | GTTCTGTCTGTTCGTGTCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.936 |
| LEAP-Seq n confirming: | 1561 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCAAGACGGTTGATGTA |
| Suggested primer 2: | GAGGGAGCTGTTGACGAGAC |