| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124978 |
| Chromosome: | chromosome 1 |
| Location: | 6769622 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048701 | (1 of 3) IPR002913 - START domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAGCAAAGAAGGGGACAGGTACGGTA |
| Internal bar code: | TCGTCGGCTCATCGTTGCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 99.5525 |
| LEAP-Seq n confirming: | 5339 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCTGTTTTCACCATTT |
| Suggested primer 2: | ACGTGTGGTACGTCAACGAG |