| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124990 |
| Chromosome: | chromosome 7 |
| Location: | 455022 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315450 | CSG2 | Predicted protein of CSG family | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGGCGGATGTCATGGATTAACGCCACA |
| Internal bar code: | AGCTTGTTTGGACTAGGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 986 |
| LEAP-Seq percent confirming: | 98.8702 |
| LEAP-Seq n confirming: | 3063 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTGTATGCCATGGAAGC |
| Suggested primer 2: | ACTCCCACCGTTTTACCTCC |