Insertion junction: LMJ.RY0402.125001_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.125001
Chromosome:chromosome 2
Location:3089088
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTAATCCGATGTCCGTATCGATCGCCACG
Internal bar code:AATCGCCCTTGGTCGTCAAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:948
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTATCAACGAGGACGGCAAT
Suggested primer 2:AGGCATCATTTCCAGCAAAC