| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125001 |
| Chromosome: | chromosome 7 |
| Location: | 1914505 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325744 | (1 of 1) PTHR19277//PTHR19277:SF102 - PENTRAXIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGCGGGAGTCAAGGCAGCGAGGCGGT |
| Internal bar code: | GGAGACGCGTGGCAGGCTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 96.9673 |
| LEAP-Seq n confirming: | 6075 |
| LEAP-Seq n nonconfirming: | 190 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAAGGAAAACACGCATA |
| Suggested primer 2: | ATTGAGCACTCGTGCCTCTT |