| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125023 |
| Chromosome: | chromosome 3 |
| Location: | 7337942 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206600 | AAD1 | Acetohydroxyacid dehydratase; (1 of 1) 4.2.1.9 - Dihydroxy-acid dehydratase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCAAACGCCTGGGCACAAGGGCTGATT |
| Internal bar code: | CCGTTTGGGGCTGTAACCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.714 |
| LEAP-Seq n confirming: | 14994 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGCTTGATGGGCTTCTC |
| Suggested primer 2: | CGGTTGGTGGGAGAAGACTA |