| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125053 |
| Chromosome: | chromosome 9 |
| Location: | 4619771 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396549 | (1 of 12) IPR001841//IPR002110//IPR020683 - Zinc finger, RING-type // Ankyrin repeat // Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCGCTGGAATGTGCTCGTCTTCTACT |
| Internal bar code: | AGCTCCCCCCCTTCGACCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 99.3333 |
| LEAP-Seq n confirming: | 745 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCTGGAGGAAGGAGAGG |
| Suggested primer 2: | AACACCGTTTGAGGTTCGAC |