| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125057 |
| Chromosome: | chromosome 12 |
| Location: | 7922382 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552650 | PAP8 | (1 of 1) PTHR23092:SF19 - NUCLEOTIDYLTRANSFERASE; Class-II RNA nucleotidyl transferase 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCAGGCAGGCTGCCGGCGCTTCCTCT |
| Internal bar code: | GCGGGGCGGGCAATCAATTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 48.2759 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 480 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTCCACGAGAGTTAGG |
| Suggested primer 2: | CGAGTACTACCTTGCGCTCC |