| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125069 |
| Chromosome: | chromosome 6 |
| Location: | 2226589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266300 | (1 of 15) PTHR12291//PTHR12291:SF23 - FAMILY NOT NAMED // PROTEIN MEC-15 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACGGACCCAGAGGCACAGCGCTTGGCC |
| Internal bar code: | AATGGGAACGCGCCTCGAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 378 |
| LEAP-Seq percent confirming: | 99.7924 |
| LEAP-Seq n confirming: | 3846 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTATACGGTTCCGCTGCT |
| Suggested primer 2: | CACTCCCCTTCCTTTCTTCC |