Insertion junction: LMJ.RY0402.125072_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.125072
Chromosome:chromosome 7
Location:3954111
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g339500 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAAGAACTGCAGGCATGCACAACAGGCA
Internal bar code:TGTTTTAGCGGAAGCGCTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.105932
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1886
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATTTTGATTGCGCCACTTT
Suggested primer 2:CAAACTGTGTGGGTCACTGG