| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125075 |
| Chromosome: | chromosome 6 |
| Location: | 122615 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249950 | (1 of 3) K00889 - 1-phosphatidylinositol-4-phosphate 5-kinase (PIP5K) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGGCGGCCGCACGCGGCTGGCTGGCC |
| Internal bar code: | GGGGAATCGACAGGGTGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 107 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTGTCACTATGGGATGC |
| Suggested primer 2: | AGCTGCCACATAGGGCTGTA |