| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125110 |
| Chromosome: | chromosome_6 |
| Location: | 2390501 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g267850 | PLS1 | Phospholipid scramblase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGAGTGTGCAGGCGGTGTGGAGCGGGCG |
| Internal bar code: | AACTCGCCCGAGGGGGCCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 288 |
| LEAP-Seq percent confirming: | 97.002 |
| LEAP-Seq n confirming: | 1456 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATCCACACCCCCTTCCTC |
| Suggested primer 2: | AGGAGTGGTAGGCGTTGATG |