| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125118 |
| Chromosome: | chromosome 1 |
| Location: | 6811221 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049100 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCTTCGGCGTGTGGGCACGCGGCGTTG |
| Internal bar code: | GCAGGAGAGACAACGGTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 98.4728 |
| LEAP-Seq n confirming: | 1612 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGATGGTGTGCGTGACAT |
| Suggested primer 2: | CTTTGTTGTGGAGGTCGGTT |