Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.125125 |
Chromosome: | chromosome 14 |
Location: | 1199926 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g616000 | (1 of 35) IPR001763 - Rhodanese-like domain | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATGCATGACCACAGTTGCATTACATGA |
Internal bar code: | ATTATAGTAAAATATACCTCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 151 |
LEAP-Seq percent confirming: | 99.2188 |
LEAP-Seq n confirming: | 254 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCGTTTAGGTTGAGTGGG |
Suggested primer 2: | TACTCTCACTTGGTGCCGTG |