Insertion junction: LMJ.RY0402.125137_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125137
Chromosome:chromosome 7
Location:3642625
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g337100 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTGATGGTGAGGCGCCAGTGACCTCAAC
Internal bar code:AGGAGTGTCCCGCACTAGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1021
LEAP-Seq percent confirming:99.8395
LEAP-Seq n confirming:6841
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGATAAGCTTAGGGCGAT
Suggested primer 2:GCTTGGTGGAGACGTTTGTT