| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125149 |
| Chromosome: | scaffold 24 |
| Location: | 56040 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755497 | (1 of 2) K05770 - benzodiazapine receptor (BZRP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCACGATTCTCGCTTGAGCTTTTGAG |
| Internal bar code: | TCGTTATATCTCGCTTTCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 99.7706 |
| LEAP-Seq n confirming: | 1305 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCATGAATGTACCCCC |
| Suggested primer 2: | GGAAAGCAGCAAAGATGGAG |