| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125154 |
| Chromosome: | chromosome 16 |
| Location: | 1166542 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650500 | (1 of 1) K06195 - ApaG protein (apaG) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCATCGTGCCGAACTACCGCACGCGGGT |
| Internal bar code: | GAAACGTACGGGCTCTGCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 770 |
| LEAP-Seq percent confirming: | 81.1074 |
| LEAP-Seq n confirming: | 2417 |
| LEAP-Seq n nonconfirming: | 563 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTGGTTCTGGATCTTGC |
| Suggested primer 2: | ACCTAGACCGCACAAAATGG |