| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125168 |
| Chromosome: | chromosome 1 |
| Location: | 795968 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004124 | (1 of 3) 3.2.1.8 - Endo-1,4-beta-xylanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTGTAACAGAATGCGCCTTGATAGCAC |
| Internal bar code: | TCTTTTGTATGCAACGGGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 97.8038 |
| LEAP-Seq n confirming: | 668 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTTGGCTAGCTTGTTTG |
| Suggested primer 2: | ATGAAGATGCCCTCGTTGAG |