| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125174 |
| Chromosome: | chromosome_10 |
| Location: | 4631368 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g452950 | TRP1 | Transient receptor potential ion channel protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTGCACTGCACGCTGTTGCACCCGCCCCA |
| Internal bar code: | TAGCGTTTCTTTAGCTCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 99.8042 |
| LEAP-Seq n confirming: | 1529 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTGTACGGGTTGTACG |
| Suggested primer 2: | GATAGAGCTGCTACACCCCG |