| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125195 |
| Chromosome: | chromosome_1 |
| Location: | 6191656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g044276 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTAGCAGGGAGCAACTGTTGTGATGCGGA |
| Internal bar code: | CGAATCGTTTCGTAAGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 840 |
| LEAP-Seq percent confirming: | 99.6953 |
| LEAP-Seq n confirming: | 3926 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCTGCACAGCAGTCGTT |
| Suggested primer 2: | CTTTGAAAGCATGCCACAGA |