| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125197 |
| Chromosome: | chromosome 12 |
| Location: | 9525335 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543902 | (1 of 1) 2.7.1.16 - Ribulokinase / L-ribulokinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAAGCACGGGCGGGGCCACGTGTTCCG |
| Internal bar code: | TGCATACCATGAATGGCGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 98.6399 |
| LEAP-Seq n confirming: | 4714 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGATATCATGGACCGT |
| Suggested primer 2: | TGAGCGTTAGTATGGCGATG |