| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125200 |
| Chromosome: | chromosome 12 |
| Location: | 3320526 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497750 | TSPP1,TPS2 | Trehalose 6-phosphate phosphatase; (1 of 1) K01087 - trehalose 6-phosphate phosphatase (otsB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATGAATGCGGCTGTGGCGACAATTTTG |
| Internal bar code: | CCGCGCCTGGGAGAACGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.8964 |
| LEAP-Seq n confirming: | 6753 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACCTGAACACAACCGTG |
| Suggested primer 2: | CCACGATAATGTGTGAACGC |