| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125205 |
| Chromosome: | chromosome_11 |
| Location: | 3640775 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g482276 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTGGACAAGTACGCGCACCCGGTGGTGTA |
| Internal bar code: | GCAGTAGACAAGACGTGCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 91.7808 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCCATACTCGGGCTCA |
| Suggested primer 2: | GGTTATCTGGCGCTAAGCAG |