Insertion junction: LMJ.RY0402.125222_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.125222
Chromosome:chromosome 7
Location:4109482
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g342920 (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGTGTCCGTACGGCACCGCCGCCGTACA
Internal bar code:GTATGGACCTCCTTAACTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:120
LEAP-Seq percent confirming:99.1176
LEAP-Seq n confirming:337
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACCTGTCCGTGTTTC
Suggested primer 2:AAACTGAATCGAACCCAACG