Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.125247 |
Chromosome: | chromosome 17 |
Location: | 5514237 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCACCCGTACGAGGCTTTGGCATCACA |
Internal bar code: | GTAGCGATGCCTGGTCCGTGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1 |
LEAP-Seq percent confirming: | 99.8782 |
LEAP-Seq n confirming: | 3279 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCAAACTGGGACCTCTACA |
Suggested primer 2: | TGATCTGTTCCTGTTCGCTG |