| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125252 |
| Chromosome: | chromosome 5 |
| Location: | 840162 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247900 | (1 of 13) PF00646 - F-box domain (F-box) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACCACCCCACCACTCTGGCCGGCGCCGC |
| Internal bar code: | CGGTGAAGTCTGTCGCAACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 61.4811 |
| LEAP-Seq n confirming: | 3877 |
| LEAP-Seq n nonconfirming: | 2429 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAATTGACGACCCACACA |
| Suggested primer 2: | CCGGAAGTTACCTCACCGTA |