| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125254 |
| Chromosome: | chromosome 16 |
| Location: | 3715477 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688901 | CYG56 | (1 of 1) PTHR11920//PTHR11920:SF345 - ADENYLATE AND GUANYLATE CYCLASES // SOLUBLE GUANYLATE CYCLASE 88E; NO-dependent guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGACACGACACAGTGCACGCAACCTCCA |
| Internal bar code: | ATTGGCTGACAGCCGGTCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 99.4383 |
| LEAP-Seq n confirming: | 5311 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTCGACACTGTCTTCAT |
| Suggested primer 2: | TACCAGCCCAAGATAATCCG |